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現(xiàn)代組織化學(xué)技術(shù)和方法

現(xiàn)代組織化學(xué)技術(shù)和方法

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作 者: 周勁松 編
出版社: 西安交通大學(xué)出版社
叢編項(xiàng): 研究生創(chuàng)新教育系列教材
標(biāo) 簽: 暫缺

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ISBN: 9787560555614 出版時(shí)間: 2015-04-01 包裝: 平裝
開本: 16開 頁(yè)數(shù): 233 字?jǐn)?shù):  

內(nèi)容簡(jiǎn)介

《現(xiàn)代組織化學(xué)技術(shù)和方法/研究生創(chuàng)新教育系列教材》的主要內(nèi)容包括組織標(biāo)本的前期制備技術(shù)和方法,組織化學(xué)基本概念和理論以及糖、核酸、脂和酶的染色方法,免疫組織化學(xué)基本概念和理論以及染色方法,組織化學(xué)與免疫組織化學(xué)染色技術(shù)與凝集素染色技術(shù)、PCR、原位雜交和電鏡技術(shù)的結(jié)合。在《現(xiàn)代組織化學(xué)技術(shù)和方法/研究生創(chuàng)新教育系列教材》最后,著重介紹了目前比較先進(jìn)的定量檢測(cè)儀器的基本理論和使用方法,以及與本領(lǐng)域有關(guān)的最近研究進(jìn)展。

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暫缺《現(xiàn)代組織化學(xué)技術(shù)和方法》作者簡(jiǎn)介

圖書目錄

Chapter 1 Introduction
Section 1 The contents and theories in Histochemistry
Section 2 The basic requirements of Histochemistry methods

Chapter 2 The Tissue Preparation
Section 1 Tissue collection
Section 2 Fixation
Section 3 Tissue washing-up, dehydration and clearance
Section 4 Embedding
Section 5 Sectioning
Section 6 Adherence and mounting
Section 7 Buffer

Chapter 3 The Carbohydrate and its Derivatives Histochemistry
Section 1 Classification
Section 2 Histochemistry methods

Chapter 4 The Nucleic Acid Histochemistry
Section 1 DNA Histochemical demonstration
Section 2 Comparison demonstration of DNA and RNA Histochemistry

Chapter 5 The Lipid Histochemistry
Section 1 Overview
Section 2 Demonstration of lipids with physical methods
Section 3 Demonstration of lipids with chemical methods

Chapter 6 Enzyme Histochemistry
Section 1 Enzyme and its basic histochemical theory
Section 2 Histochemistry for common enzymes

Chapter 7 The Basic Theory of Immunohistochemistry
Section 1 Basic Immunology
Section 2 Common markers and their detection
Section 3 Basic conditions

Chapter 8 Common Methods Used in Immunohistochemistry
Section 1 Principles
Section 2 Immunofluorescence method
Section 3 Immunoenzyme technique
Section 4 Avidin-biotin method
Section 5 Protein A method
Section 6 Immunogold method and immuno gold-silver method

Chapter 9 Specificity and Sensitivity of Immunohistochemistry
Section 1 Specificity and specific staining
Section 2 Control experiment
Section 3 The methods to enhance immunohistochemical sensitivity

Chapter 10 The Double-Staining in Immunohistochemistry
Section 1 Double-staining Immunohistochemistry on serial section
Section 2 Immunofluorescence double labeling technique
Section 3 Immunoenzyme double staining technique
Section 4 Immunoenzyme-Immunofluorescence double staining
Section 5 Immunoenzyme-Immunogold double staining

Chapter 11 The Lectin Histochemistry
Section 1 Characteristics and application of lectin
Section 2 Application of lectin Histochemistry

Chapter 12 The Progress of in Situ Display
Section 1 The envision method
Section 2 The catalyzed signal amplification method
Section 3 The ferric oxide alternative method for HRP

Chapter 13 In Situ Hybridization Immunohistochemistry
Section 1 Basic principle
Section 2 Probe preparation
Section 3 Procedure of in situ hybridization histochemistry
Section 4 Factors affecting in situ hybridization
Section 5 Control test

Chapter 14 In Situ Polymerase Chain Reaction Technique
Section 1 Basic principle
Section 2 Basic types
Section 3 Procedure
Section 4 Application of in situ PeR technology

Chapter 15 Electron Microscopic Cytochemical Technique
Section 1 Electron microscopic enzyme cytochemistry technology
Section 2 Electron microscopic Immunocytochemical technique

Chapter 16 The Quantitative Assay of Histochemistry Results
Section 1 The photomicrography
Section 2 The image analyzer
Section 3 The flow cytometry
Section 4 The laser scanning confocal microscopy
References
Appendix I Preparation of Commonly Used Buffer
Appendix II Histochemistry and Immunohistochemistry Experiments
Appendix III Figures and pictures

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