Chapter 1 Introduction 1.1 The nature of biotechnology 1.2 Historical evolution of biotechnology 1.3 Application of biotechnology 1.4 Growth in biotechnology 1.5 Strategic planning in biotechnology 1.6 Summary Chapter 2 Biochemistry of Growth Metabolism 2.1 Introduction 2.2 Catabolism and energy 2.3 Catabolic pathways 2.4 Gluconeogenesis 2.5 Energy metabolism in aerobic organisms 2.6 Energy production in anaerobic organisms 2.7 Anaerobic metabolism 2.8 Biosynthesis and growth Chapter 3 Applied Genetics 3.1 Selection and screening 3.2 Culture maintenance 3.3 Mutagenesis 3.4 Sexual hybridization 3.5 Parasexual processes 3.6 Recombinant DNA technology 3.7 Regulation and control of recombinant DNA experimentation 3.8 Summary Chapter 4 Fermentation Technology 4.1 The nature of fermentation 4.2 Principles of microbial cultivation in aqueous systems 4.3 Bioreactor design 4.4 Media design 4.5 Instrumentation and process control in bioreactors 4.6 Measurement techniques 4.7 Mass and energy transfer 4.8 Scale_up 4.9 Solid substrate fermentation 4.10 Summary Chapter 5 Enzyme and Immobilized Cell Technology 5.1 The commercial role of isolated enzymes 5.2 Sources of enzymes 5.3 Production of enzymes 5.4 Enzyme legislation 5.5 Immobilized enzymes 5.6 Properties of immobilizde enzymes 5.7 Immobilization of cells 5.8 Summary Chapter 6 Downstream Processing in Biotechnology 6.1 Introduction 6.2 Separation of particles 6.3 Disintegration of cells 6.4 Extraction methods 6.5 Concentration methods 6.6 Purification and resolution of mixtures 6.7 Drying Chapter 7 Instrumentation 7.1 Introduction 7.2 Terminology 7.3 Process control 7.4 Air flow moniroring 7.5 Measurements of power input 7.6 Temperature measurements 7.7 Rheological measurements 7.8 Foam control 7.9 pH probes 7.10 Redox probes 7.11 Dissolved oxygen probes 7.12 Enzyme probes 7.13 Gas analysis 7.14 Determination of cell concentration Chapter 8 Processes and Products Dependent on Cultured Animal Cells 8.1 Historical 8.2 Types of products which can be obtained from cultured animal cells 8.3 Overview of methodology for product generation 8.4 Monolayer cell growth systems 8.5 Doownstream processing 8.6 Genetically engineered animal cells and bacteria Chapter 9 Products from Plant Cells 附錄
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